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After 6 h, the animals were anesthetized with ketamine/xylazine and sacrificed by peritoneal exudates, which have been washed with three mL of cold PBS. The total cell numbers were determined in a Neubauer chamber after staining with Turk’s resolution, after exudates werecentrifugationd at 250 g for 10 minutes. The supernatants have been collected and used…
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